How to Process Samples for Metabolomics in Ecology: A Comparative Study of Preanalytical Storage Methods.

ABSTRACT: The technical progress of metabolomics-the analysis of metabolite composition of cells, tissues, and biofluids-and its ability to unravel phenotypical responses of organisms to their environment was accompanied by the democratization of their use in a wide range of scientific fields. While the reliability of analytical procedures was thoroughly assessed, the effects of preanalytical processing on sample metabolic profiles (i.e., conditioning, storage, transportation, etc.) remain quite unclear. This uncertainty is especially notable for samples collected in the frame of ecological studies, which often involve field sampling and remote sample production, leading to extended transportation durations and suboptimal storage conditions. In this study, we evaluated the impact of storage duration

SUBMITTER: Le Bris N 

PROVIDER: S-EPMC12690189 | biostudies-literature | 2025 Dec

REPOSITORIES: biostudies-literature