A second genetic screen for gurken mRNA mislocalisation uncovers novel phenotypes of piRNA pathway mutants in Drosophila.

ABSTRACT: Transposon silencing is essential for germline development. In Drosophila oogenesis, DNA damage caused by transposon activation affects microtubule-dependent mRNA localisation in the oocyte and impairs embryonic axes formation. Our previous EMS mutagenesis screen for gurken mRNA mislocalisation on chromosome 3L of Drosophila melanogaster identified several piRNA pathway mutants ( Hayashi et al., 2014). Here, we report the screen for chromosome 3R. We identified ten mutation groups disrupting gurken mRNA localisation and other mutations affecting different aspects of oogenesis. We found that mutations in karyopherin-β3 affect localisation and translation of gurken mRNA in a transposon silencing-independent manner. Characterisation of the new mutation in vreteno revealed that the piRNA pathw

SUBMITTER: Liddell SJ 

PROVIDER: S-EPMC12690538 | biostudies-literature | 2025 Nov

REPOSITORIES: biostudies-literature