PEGylation-Driven Remodeling of the Protein Corona on PLGA Nanoparticles: Implications for Macrophage Recognition.

ABSTRACT: The formation of a Protein Corona (PC) on the surface of nanoparticles (NPs) is a critical event that shapes their biological identity and governs interactions with the immune system. In this study, we investigated the composition of the PC formed on mixtures of PLGA and PEG-PLGA NPs, aiming to elucidate the link between NPsurface chemistry, proteomic fingerprint in cell culture medium, and uptake by bone marrow-derived macrophages (BMDMs). NPs showed different sizes but comparable actual PEG amount exposed on the surface, which is significantly lower than the theoretical values. The PC, isolated using a standardized microfiltration protocol, revealed distinct patterns of protein adsorption as a function of the PEG density. Uptake studies in BMDMs revealed a strong inverse relationship bet

SUBMITTER: Spinelli L 

PROVIDER: S-EPMC12690586 | biostudies-literature | 2025 Dec

REPOSITORIES: biostudies-literature