Simulation Insights into the Assembly of Polyplexes for RNA Delivery.

ABSTRACT: RNA-based pharmaceuticals proved successful with the COVID-19 vaccines and are now undergoing clinical trials for a broad range of therapeutic indications. Lipid-based nanoparticles (LNPs) have been used so far as delivery systems, although alternatives are still needed to meet efficacy and safety requirements across a broader range of applications. Polyplexes, formed by the self-assembly of cationic polymers with the anionic nucleic acids, constitute a valuable substitute, especially if precise control of the number and shape of the encapsulated RNA chains is possible. Here, we use molecular dynamics simulations of a coarse-grained polyplex model to show that the most important factors controlling it are the charge ratio between polyelectrolytes and RNA and their concentration during asse

SUBMITTER: Lehnen JH 

PROVIDER: S-EPMC12690597 | biostudies-literature | 2025 Dec

REPOSITORIES: biostudies-literature