Heterologous biosynthetic crosstalk with the native mansouramycin cluster in <i>Streptomyces albus</i> Del14 reveals unexpected metabolites.

ABSTRACT: Streptomyces albus J1074 (now S. albidoflavus J1074) is a widely used heterologous host for natural product discovery due to its capacity to express biosynthetic gene clusters (BGCs) from diverse organisms. A derivative of this strain, S. albus Del14, enhances heterologous expression by reducing background metabolite production enabling the identification of the previously hidden BGC responsible for producing mansouramycins. In this study, we demonstrate the biosynthetic crosstalk between the native mansouramycin BGC in S. albus Del14 and introduced BGCs from three different organisms results in the production of novel compounds, some featuring rare and complex chemical scaffolds. These include malevonin, which combines NRPS- and mansouramycin-derived building b

SUBMITTER: Stierhof M 

PROVIDER: S-EPMC12690601 | biostudies-literature | 2026 Feb

REPOSITORIES: biostudies-literature