Mitochondrial superoxide dismutase controls metabolic plasticity in pancreatic cancer.

ABSTRACT:

Background

The role of reactive oxygen species (ROS) in cancer is debated. One main antioxidant enzyme is mitochondrial superoxide dismutase (SOD2) which has been shown to influence tumor initiation and metastatic progression in several cancer types.

Methods

To investigate the impact of Sod2 deletion on pancreatic cancer biology and metabolism, we used CRISPR/Cas9 gene editing to generate 3 independent Sod2-deficient cell lines from murine Kras^G12D pancreatic cancer cell lines and analyzed them for proliferation, colony forming, mitochondrial respiration and RNA expression. In addition, mass spectrometry and isotope tracing were performed.

Results

Proliferation and wound healing capacity were significantly impaired in Sod2 deficient cell lines. Myc levels w

SUBMITTER: Ramasubramanian S 

PROVIDER: S-EPMC12690781 | biostudies-literature | 2025 Dec

REPOSITORIES: biostudies-literature