Plasma Fibronectin Drives Macrophage Elongation via Integrin β3-Tie2 Axis in Blood Clots.

ABSTRACT: Background/Objectives: Plasma fibronectin (pFN) supports lung metastasis by promoting tumor cell invasion and survival in the context of blood clotting. Here, we set out to test if myeloid cells reiterate the clot-invasive mechanisms that have been established for tumor cells. Methods: We analyzed lung tissue sections from transgenic pFN-deficient mice for the co-localization of intravenously injected B16F1 tumor cells and the surrounding fibrin with myeloid cells, granulocytes, and macrophages. We also tested the role of pFN for macrophage differentiation and invasion in a three-dimensional fibrin matrix. Results: B16F1 melanoma cells, entrapped in the lungs of pFN-competent C57BL/6-Fn(fl/fl)Mx-Cre^- mice, were surrounded by a fibrin mat

SUBMITTER: Knowles LM 

PROVIDER: S-EPMC12691047 | biostudies-literature | 2025 Nov

REPOSITORIES: biostudies-literature