Molecular Epidemiology and Antibiotic Resistance of Sheep-Derived <i>Mannheimia haemolytica</i> in Northwestern China.

ABSTRACT: This study comprehensively explored the epidemiology, virulence, antibiotic resistance, and genomic features of ovine-derived Mannheimia haemolytica across three provinces in Northwest China (Gansu, Ningxia, and Shaanxi). Lung tissue samples were collected postmortem from sheep with respiratory disease (nine per province), cultured under sterile conditions, and screened by morphological, Gram staining, PCR, and NGS methods. Nine M. haemolytica strains were successfully isolated (isolation rate 33.33%) and identified as serotypes A1, A2, and A6, with Shaanxi exhibiting a significantly higher isolation rate of 66.67%. Genomic analysis revealed that each isolate each harbored over 100 virulence genes and displayed notable genetic diversity. In murine assays, intraperitoneal inoc

SUBMITTER: Wang C 

PROVIDER: S-EPMC12691142 | biostudies-literature | 2025 Dec

REPOSITORIES: biostudies-literature