Functional Division of Insect Blood Cells by Single-Cell RNA-Sequencing and Cell-Type-Specific FISH Markers.

ABSTRACT: Hemocytes (insect blood cells) consist of several morphological types and perform a variety of physiological processes, including immune responses. However, we do not know how many cell types are functionally differentiated in hemocytes or how they perform independent physiological processes. To address this fundamental question, we analyzed hemocyte transcripts with a single-cell RNA-sequencing (scRNA-Seq) technique. The hemocytes were collected from larvae of a lepidopteran insect, Spodoptera exigua, in which four different hemocyte types were morphologically recognized. scRNA-Seq discriminated 24 hemocyte clusters based on the transcripts of each cell. The clusters were separated into seven functional groups predicted from the top three highly expressed and annotated genes in eac

SUBMITTER: Khan F 

PROVIDER: S-EPMC12691282 | biostudies-literature | 2025 Nov

REPOSITORIES: biostudies-literature