Manipulating PARK7/DJ-1 Levels by Genotoxic Stress Alters Noncoding RNAs and Cellular Homeostasis.

ABSTRACT: DJ-1/PARK7 is a multifunctional protein that plays a vital role in sensing oxidative stress and maintaining redox homeostasis. As an oncogene, DJ-1 influences p53-mediated stress responses and contributes to cancer progression. This study investigates the impact of X-ray-induced DNA breaks on cellular responses under varying DJ-1 expression levels. Using siRNA knockdown and overexpression approaches, transcriptional changes were analyzed by RNA-seq. Naïve cells exhibited only a moderate response to X-ray exposure, including suppression of the cell cycle and activation of stress pathways. In contrast, DJ-1 overexpression caused pronounced gene-expression suppression, particularly affecting ribosomal genes and mitochondria, with 21- and 3.5-fold enrichment, respectively. DJ-1 knockdown led t

SUBMITTER: Zohar K 

PROVIDER: S-EPMC12691451 | biostudies-literature | 2025 Nov

REPOSITORIES: biostudies-literature