Small RNAs positively and negatively control transcription elongation through modulation of Rho utilization site accessibility.

ABSTRACT: Bacteria use a multi-layered regulatory strategy to precisely and rapidly tune gene expression in response to environmental cues. Small RNAs (sRNAs) form an important layer of gene expression control and most act post-transcriptionally to control translation and stability of mRNAs. We have shown that at least five different sRNAs (RydC, CpxQ, ArrS, GcvB, and OxyS) in Escherichia coli regulate the cyclopropane fatty acid synthase (cfa) mRNA. These sRNAs bind at different sites in the long 5' untranslated region (UTR) of cfa mRNA, and previous work suggested that they modulate RNase E-dependent mRNA turnover. Rho-dependent transcription termination in the cfa 5'' UTR was demonstrated, leading us to hypothesize that the sRNAs might also regulate cfa transcri

SUBMITTER: Farley KR 

PROVIDER: S-EPMC12691595 | biostudies-literature | 2025 Dec

REPOSITORIES: biostudies-literature