Mutations in two-component signaling systems drive experimental evolution of tigecycline and colistin resistance in <i>Acinetobacter baumannii</i>.

ABSTRACT: The treatment of infections by Acinetobacter baumannii, a clinically significant nosocomial gram-negative bacterial pathogen, is hampered by antibiotic resistance, which is exacerbated by its exceptional genetic plasticity. Knowledge of the dynamics and mechanisms underlying the acquisition of antibiotic resistance is essential for the proper stewardship of their utilization. Here, we used a continuous culture device (morbidostat) to characterize the evolutionary trajectories of two A. baumannii strains in response to the increasing pressure of last-resort drugs, tigecycline and colistin. This approach allows us to confidently and comprehensively map resistance-driving mutations while circumventing both the "driver vs passenger" uncertainty and "selection bottleneck" limitati

SUBMITTER: Kent JE 

PROVIDER: S-EPMC12691633 | biostudies-literature | 2025 Dec

REPOSITORIES: biostudies-literature