Optimizing antimicrobial susceptibility testing: cost and environmental benefits of MIC volume reduction.

ABSTRACT: The determination of minimum inhibitory concentrations (MICs) is essential for evaluating antimicrobial efficacy, guiding both clinical treatment decisions and drug development. The standard broth microdilution method is widely used but requires significant reagent volumes, which can be limiting when working with novel or expensive antimicrobials. This study assesses the feasibility of reducing assay volumes without compromising MIC accuracy. We compared the MIC values obtained in standard 96-well plates (100 and 200 µL) to those in 384-well plates with reduced volumes (30 and 50 µL) for a range of ATCC gram-negative and gram-positive bacteria, as well as yeast species. Our results demonstrate that, except for micafungin against yeast, MIC values obtained with reduced volumes remained with

SUBMITTER: Clarhaut J 

PROVIDER: S-EPMC12691650 | biostudies-literature | 2025 Dec

REPOSITORIES: biostudies-literature