The essential role of 2,4-dienoyl-CoA reductase for the degradation of complex fatty acid mixtures.

ABSTRACT: Fatty acids (FAs) can be used as a carbon and energy source by most bacteria. FAs are diverse and show variations in aliphatic chain length, degree and kind of branching, and number of double bonds. After activation by a thioester link to coenzyme A (CoA), FAs are degraded by the β-oxidation machinery. The core β-oxidation enzymes can degrade most FAs, except those that bear an unsaturation at even-numbered carbons. Such FAs include the essential arachidonic and linoleic FAs of the mammalian diet. We studied the role of the 2,4-dienoyl-CoA reductase FadH in E. coli FA metabolism. We showed that fadH is essential for growth on linoleic acid and that cysteine residues connecting the FadH-bound [Fe-S] cluster are essential for activity in vivo. Eukaryotes also use 2,4-die

SUBMITTER: Schiaffi V 

PROVIDER: S-EPMC12691678 | biostudies-literature | 2025 Dec

REPOSITORIES: biostudies-literature