PRSS38 Is a Novel Sperm Serine Protease Involved in Human and Mouse Fertilization.

ABSTRACT: Sperm proteases are involved in several gamete interaction events leading to fertilization. This report presents a detailed analysis of the expression and localization of serine protease PRSS38 in human and in mouse spermatozoa and its involvement in fertilization-related events, using bioinformatics, cellular, biochemical, molecular, and functional approaches. Bioinformatics analyses included genomics and data analysis, prediction of protein subcellular localization and post-translational modifications, Self-Organizing Maps (SOMs) unsupervised training with other serine proteases, protein modeling (AlphaFold), and genetic variant analysis. For cellular, biochemical, and functional studies, human semen samples were obtained from healthy normozoospermic volunteers, and cauda epididym

SUBMITTER: Manjon AA 

PROVIDER: S-EPMC12691765 | biostudies-literature | 2025 Dec

REPOSITORIES: biostudies-literature