Characterization of the Regulatory AAA-ATPase Subunit Rpt3 in <i>Plasmodium berghei</i> as an Activator of Protein Phosphatase 1.

ABSTRACT: The 26S proteasome is the main proteolytic machinery involved in protein degradation, thereby contributing to the homeostasis and stress response of eukaryotic cells. This macromolecular complex consists of a 20S core particle assembled with one or two 19S regulatory particles. Here, we describe the Plasmodium berghei (Pb) proteasome AAA-ATPase regulatory subunit Rpt3 and demonstrate its binding to the Protein Phosphatase 1 catalytic subunit (PP1c), which is one of the major and essential parasite phosphatases. The PbRpt3 protein enhances the activity of PP1c both in vitro and in a Xenopus oocyte heterologous model. Further investigation of this model suggests that the PbRpt3-PP1c interaction may occur outside of the proteasome, and it reveals that the RVxF motifs of PbRpt3 a

SUBMITTER: Laine C 

PROVIDER: S-EPMC12691818 | biostudies-literature | 2025 Dec

REPOSITORIES: biostudies-literature