Genomic Characterization and Resistance Mechanisms of Carbapenem-Resistant <i>Klebsiella pneumoniae</i> ST101 Isolates from Saudi Arabia.

ABSTRACT: Carbapenem-resistant Klebsiella pneumoniae (CRKP) represents a critical global health threat, with ST101 identified as a major circulating clone in Saudi Arabia. We used whole genome sequencing and plasmid reconstruction to investigate the molecular characteristics of CRKP ST101 isolates from Saudi Arabia (2018-2021), analyzing antimicrobial resistance genes (ARGs), virulence factors, and plasmid structure and replicon types. Clinical isolates were obtained from the Ministry of National Guard Health Affairs (MNGHA) hospitals in Saudi Arabia between 2018 and 2021. Whole-genome sequencing was performed using the Illumina MiSeq^® platform, followed by comprehensive bioinformatic analysis of ARGs, virulence factors, and plasmid content. All ten isolates belonged to ST101 and h

SUBMITTER: Idrees EK 

PROVIDER: S-EPMC12691828 | biostudies-literature | 2025 Nov

REPOSITORIES: biostudies-literature