Gold Nanoparticles as a Possible Tool to Untangle Some Structural Features of the Gluten Network.

ABSTRACT: Aqueous semolina suspensions were reacted with spherical gold nanoparticles (AuNPs, nominal diameter, 20 nm) to assess the accessibility of cysteine thiols in durum wheat proteins, focusing on network-forming gluten proteins. Unlike small thiol reagents, covalent bond formation between gold ions on the AuNPs surface and protein thiols was greatly facilitated by the addition of 1% sodium dodecyl sulfate (SDS). SDS weakens non-covalent hydrophobic interactions within and among proteins, increasing the exposure of buried thiols without altering disulfide bonds. MS/MS analysis of proteolytic fragments from the isolated AuNP-protein covalent complexes allowed identification of the bound proteins. Proteomics data suggests that AuNPs also associate with gluten proteins lacking free thiols in thei

SUBMITTER: Emide D 

PROVIDER: S-EPMC12691966 | biostudies-literature | 2025 Nov

REPOSITORIES: biostudies-literature