Enhancing Secondary Metabolite Production in Actinobacteria Through Over-Expression of a Medium-Sized SARP Regulator.

ABSTRACT: Natural products hold immense therapeutic potential, yet they remain underexplored due to challenges in activating or producing them in laboratory settings. Here, we investigate the regulatory capabilities of a new medium-sized Streptomyces Antibiotic Regulator Protein (SARP), Fzm_SARP, in comparison to the well-characterized small SARP, RedD, across 18 diverse actinobacterial strains. In addition to the conserved DNA-binding domains typical of SARP regulators, the medium-sized Fzm_SARP also contains an additional NTPase domain. Our study revealed that 327 of the 422 metabolites (77%) detected in 18 wild-type actinobacterial strains were up-regulated in the SARP over-expressing strains. Among these 422 metabolites, 55% were up-regulated in the two SARP over-expressing strains wherea

SUBMITTER: Heng E 

PROVIDER: S-EPMC12692060 | biostudies-literature | 2025 Dec

REPOSITORIES: biostudies-literature