Interplay Between DNA Polymerase, RNA Polymerase, and RNase H1 During Head-On Transcription-Replication Conflict.

ABSTRACT: Transcription-replication conflicts (TRCs) often occur in cells and cause DNA replication fork stalling. In this study, we investigated the interplay of RNA polymerase (RNAP), DNA polymerase, and RNase H1 (RH1) during head-on TRC in vitro with precise control over the reaction conditions. We show that it is a catalytically competent transcription elongation complex (TEC) that interferes with the action of both the Klenow fragment and full-length DNA Pol I. An incompetent RNAP complex with an R-loop stimulates the 3'→5' exonuclease activity and pauses the DNA polymerase during head-on TRC. As RNAP advances along the DNA template, elongating the RNA, the head-on TRC is slowly overcome in our model system, likely through the reassociation of the displaced DNA polymerase with the nontemplate D

SUBMITTER: Timofeyeva NA 

PROVIDER: S-EPMC12692348 | biostudies-literature | 2025 Nov

REPOSITORIES: biostudies-literature