Transcriptional Profiling of Common Carp: A Microarray-Based Framework for Aquaculture Research.

ABSTRACT: The common carp Cyprinus carpio L. is a cornerstone aquaculture species, yet transcriptome interpretation is complicated by its paleotetraploid genome and extensive alternative splicing. A species-tailored oligonucleotide microarray was developed to deliver reproducible, gene-level expression profiling. Probe design was anchored to the SPL01 reference and implemented on an Agilent platform using a gene-level strategy that collapsed transcripts to genes, selected the longest isoform, and placed 3'-anchored 60-mer probes. The workflow incorporated embedded technical controls and a standardized two-color pipeline to ensure stable measurements across arrays. Baseline functional organization of the head kidney was defined using 614 C. carpio L. -Danio rerio orthologs and co

SUBMITTER: Pluta A 

PROVIDER: S-EPMC12692531 | biostudies-literature | 2025 Nov

REPOSITORIES: biostudies-literature