Promoter Specificity and Transcription Levels Modulate <i>Trans</i>-Splicing Efficiency at the <i>mod</i>(<i>mdg4</i>) Locus in <i>Drosophila</i>.

ABSTRACT: Unlike canonical cis-splicing, trans-splicing combines exons from two distinct transcripts, creating chimeric mRNAs. One striking example is the mod(mdg4) locus in Drosophila, where all mRNAs, encompassing over 30 isoforms, are exclusively generated via trans-splicing, which integrates common constitutive exons with one of the alternative 3' exons transcribed from independent promoters. This study analyzed the roles of the promoter, exons, and introns in trans-splicing, including in the constitutive part of the mod(mdg4) gene, using a previously validated model in the heterogeneous 22A genomic locus. We found that trans-splicing efficiency was significantly greater with the mod(mdg4) promoter compared to un

SUBMITTER: Beginyazova O 

PROVIDER: S-EPMC12692577 | biostudies-literature | 2025 Nov

REPOSITORIES: biostudies-literature