Rapid Detection of Animal-Derived Components in Plant-Based Meat Alternatives Using Recombinase Polymerase Amplification.

ABSTRACT: Plant-based meat alternatives (PMAs), as an emerging food category gaining increasing popularity, face potential food safety risks and ethical concerns for vegetarians due to the illegal adulteration of animal-derived components. To address these challenges and enhance regulatory oversight, the development of a rapid, sensitive, and highly specific detection method is essential. In this study, five DNA extraction methods were evaluated and optimized to identify the most effective approach for PMA products. The optimal conditions were determined to be 60 mmol/L NaCl, 10 mmol/L Tris HCl, and a centrifugation speed of 12,000× g. Additionally, specific primers targeting four common animal-derived adulterants, namely pork, chicken, duck, and beef, were designed and screened for targeted

SUBMITTER: Sun Y 

PROVIDER: S-EPMC12692603 | biostudies-literature | 2025 Nov

REPOSITORIES: biostudies-literature