Genomic diversity and antimicrobial resistance of <i>Vibrio cholerae</i> isolates from Africa: a PulseNet Africa initiative using nanopore sequencing to enhance genomic surveillance.

ABSTRACT: Objectives. Vibrio cholerae remains a significant public health threat in Africa, with antimicrobial resistance (AMR) complicating treatment. This study leverages whole-genome sequencing (WGS) of V. cholerae isolates from Côte d'Ivoire, Ghana, Zambia and South Africa to assess genomic diversity, AMR profiles and virulence, demonstrating the utility of WGS for enhanced surveillance within the PulseNet Africa network.Methods. We analysed Vibrio isolates from clinical and environmental sources (2010-2024) using Oxford Nanopore sequencing and hybracter assembly. Phylogenetic analysis, MLST, virulence and AMR gene detection were performed using Terra, Pathogenwatch and Cloud Infrastructure for Microbial Bioinformatics platforms, with comparisons against 118 gl

SUBMITTER: Foster-Nyarko E 

PROVIDER: S-EPMC12692737 | biostudies-literature | 2025 Dec

REPOSITORIES: biostudies-literature