Deciphering HIV-1 Transcription Initiation and Elongation from Single-Molecule Imaging Data.

ABSTRACT: The stages of transcription initiation and elongation are critical in the regulation of HIV-1 gene expression. Recent single-molecule imaging in living cells has shown that HIV-1 transcription occurs across multiple time scales and plays a key role in the control of latency. However, the molecular mechanisms of HIV-1 transcription remain poorly understood due to the lack of a unified modeling framework and advanced computational methods for analyzing HIV-1 imaging data. Here, we present a general stochastic model that characterizes HIV-1 transcription dynamics and computes the distributions of initiation times and nascent RNA counts. Our results show that coordination between initiation and elongation modulates transcription dynamics and that leveraging initiation-time data enhances model

SUBMITTER: Yang X 

PROVIDER: S-EPMC12692740 | biostudies-literature | 2025

REPOSITORIES: biostudies-literature