A screen for synthetic genetic interactions with the Saccharomyces cerevisiae hrq1ΔN allele.

ABSTRACT: The Saccharomyces cerevisiae  Hrq1 helicase is a functional homolog of the disease-linked human RECQL4 enzyme and has been used as a model to study RecQ4 helicase subfamily biology. Although the motor cores of Hrq1 and RECQL4 are quite similar, these proteins display distinct N-terminal domains of unknown function. Do these domains facilitate species-specific activities by the two helicases, or do they serve common roles despite their differences in sequence and predicted structure? We probed these questions here by analyzing an N-terminal domains-truncated isoform of Hrq1 (Hrq1ΔN) both in vitro and in vivo. We found that the Hrq1 N-terminal domains houses a cryptic DNA binding site that is likely important for DNA repair because the hrq1ΔN allele phenocopies the DNA inter-strand crosslink

SUBMITTER: Shumaker KA 

PROVIDER: S-EPMC12693483 | biostudies-literature | 2025 Dec

REPOSITORIES: biostudies-literature