Dissection of the Ren6 and Ren7 powdery mildew resistance loci in Vitis piasezkii DVIT2027 using phased parental-progeny genomes and intraspecific locus graph reconstruction.

ABSTRACT: The Chinese grape accession Vitis piasezkii DVIT2027 carries 2 loci associated with powdery mildew (PM) resistance, Ren6 and Ren7, which differ in timing and strength of response to Erysiphe necator. Both loci are consistent with recognition by intracellular immune receptors. To identify the underlying nucleotide-binding leucine-rich repeat (NLR) genes, we assembled chromosome-scale diploid genomes of DVIT2027 and the susceptible V. vinifera F2-35, parents of a segregating F1 population. We integrated these assemblies with deep resequencing data from 8 F1 siblines carrying different Ren6/Ren7 combinations and generated trio-binned, parent-phased genomes for 6 progeny. This resolved both PM-resistant (PMR) and PM-susceptible (PMS) haplotypes at Ren6 and Ren7. Comparative analyses revealed e

SUBMITTER: Massonnet M 

PROVIDER: S-EPMC12693563 | biostudies-literature | 2025 Dec

REPOSITORIES: biostudies-literature