Dentinogenic Effect of BMP-7 on Wharton's Jelly Mesenchymal Stem Cells Cultured in Decellularized Dental Pulp.

ABSTRACT: Decellularized tissue scaffolds mimic the native pulp-dentin microenvironment and support the odontogenic development of stem cells. This study investigated the dentinogenic effect of Wharton's Jelly Mesenchymal Stem Cells (WJMSCs) in decellularized human dental pulp (DHDP) with bone morphogenic protein-7 (BMP-7) at three concentrations: 0 ng/mL (control), 25 ng/mL, and 50 ng/mL. The effects of BMP-7 were evaluated by histological examination, WJMSC viability using AlamarBlue, dentinogenic gene expression by qPCR, and dentinogenic protein expression by ELISA. By day 21, all three groups exhibited cell distribution along the pore surfaces of DHDP, followed by the presence of a collagen matrix in the tissue. WJMSC viability treated with 25 ng/mL and 50 ng/mL showed a statistically significan

SUBMITTER: Ahmad Shuhaimi NA 

PROVIDER: S-EPMC12693673 | biostudies-literature | 2025 Dec

REPOSITORIES: biostudies-literature