Transcriptome-Based Identification of Reference Genes for Expression Analysis in Cassava Under <i>Xanthomonas phaseoli</i> pv. <i>manihotis</i> Infection.

ABSTRACT: Reverse transcription quantitative PCR (RT-qPCR) is a powerful and widely used technique for quantifying alterations in gene expression. Cassava bacterial blight caused by Xanthomonas phaseoli pv. manihotis severely constraints cassava growth and yield. Accurate evaluation of the expression levels of genes following infection by X. phaseoli pv. manihotis is crucial for the identification of potential cassava resistance genes. In this study, thirty-two novel potential reference genes were screened from the cassava-X. phaseoli pv. manihotis transcriptome. Their expression, along with that of seven literature-reported cassava reference genes, was evaluated in two susceptible and two resistant cassava varieties at six time points post-inoculation by

SUBMITTER: Yang J 

PROVIDER: S-EPMC12693826 | biostudies-literature | 2025 Nov

REPOSITORIES: biostudies-literature