Assessment of the Isolated and Combined Impact of β-Glucan and <i>Lacticaseibacillus rhamnosus</i> on Cystic Fibrosis Gut Microbiota Using a SHIME^® System.

ABSTRACT:

Background

Cystic fibrosis (CF) is a genetic disorder that disrupts gut microbiota composition, promoting dysbiosis associated with chronic inflammation, impaired nutrient absorption, and poor clinical outcomes. While modulation of the intestinal microbiota through prebiotics, probiotics, and synbiotics has been proposed as a therapeutic strategy, clinical evidence remains limited, especially in children.

Objective

This study aimed to evaluate the impact of three supplementation strategies (a prebiotic (β-glucan), a probiotic (Lacticaseibacillus rhamnosus GG), and their synbiotic combination) on the gut microbiota and metabolic activity of a CF child faecal donor using a dynamic in vitro colonic fermentation model (SHIME^®).

Methods

Microbial c

SUBMITTER: Viteri-Echeverria J 

PROVIDER: S-EPMC12694052 | biostudies-literature | 2025 Nov

REPOSITORIES: biostudies-literature