Intrinsic mechanotransduction during apical constriction licenses lineage competence in pluripotent stem cells.

ABSTRACT: To acquire the capacity for multi-lineage differentiation, pluripotent stem cells must undergo a transition from naïve pluripotency to lineage competency. This transition requires epithelialization and changes in nuclear architecture. We sought to determine whether the cell and tissue mechanics intrinsic to epithelialization drive pluripotency progression and subsequent lineage commitment to neuroectodermal fate. We demonstrate that naïve mouse embryonic stem cells (mESCs) undergoing early differentiation in vitro recapitulate features of epithelialization in the peri-implantation epiblast, specifically apical constriction. We further demonstrate that cell contractility during apical constriction induces a distinct nuclear mechanoresponse, notably enrichment of emerin at the outer n

SUBMITTER: Hamouda MS 

PROVIDER: S-EPMC12694579 | biostudies-literature | 2025 Dec

REPOSITORIES: biostudies-literature