Identification and characterization of a Fibrillin-1 derived matrikine for cardiac regeneration and repair.

ABSTRACT: The development of regenerative strategies to repair the heart is of high importance. Our lab has shown that extracellular matrix derived from decellularized fetal myocardium promotes neonatal cardiomyocyte proliferation in vitro. The goal of this study was to identify specific peptide(s)/protein(s) in solubilized cardiac ECM responsible for this proliferative effect. We hypothesized that isolation and then treatment with one or more small synthetic peptide derived from this source could replicate the cellular response to whole solubilized ECM. Decellularized fetal and adult rat hearts were fractionated by molecular weight using SDS-PAGE and transferred to PVDF membranes. Analysis of cardiomyocytes cultured on the membranes revealed regions of enhanced cardiomyocyte proliferation. Subseque

SUBMITTER: Edmunds KJ 

PROVIDER: S-EPMC12694597 | biostudies-literature | 2025 Dec

REPOSITORIES: biostudies-literature