Late-stage peptide modification with salicylaldehyde tag enhances affinity for nuclear factor-kappa B essential modulator.

ABSTRACT: The late-stage peptide functionalization with salicylaldehyde (SA) tags is described here as a versatile design of potential Lys-engaging, reversible-covalent ligands. This approach was applied to a known binder for NEMO, a protein involved in the activation of the pro-inflammatory transcription factor NFκB. Fluorescence anisotropy screening led to the identification of SA-tagged peptides with higher affinity than the wild-type sequence.

SUBMITTER: Mason M 

PROVIDER: S-EPMC12694607 | biostudies-literature | 2026 Feb

REPOSITORIES: biostudies-literature