Molecularly Imprinted Membranes: Dual@MIPs@mbr for On-Site Detection of CA 19-9.

ABSTRACT: Dual-emission molecularly imprinted membranes (dual@MIPs@mbr) were developed as a proof-of-concept platform for the selective and instrument-free detection of the cancer biomarker carbohydrate antigen 19-9 (CA 19-9). The system integrates a ratiometric fluorescence response by embedding yellow-emitting quantum dots (y-QDs), serving as target-responsive probes, and blue-emitting carbon dots (b-CDs), acting as an internal reference, within a CA 19-9-imprinted polymeric matrix. Specific rebinding of CA 19-9 to the imprinted cavities induced selective quenching of the y-QDs while preserving the b-CDs emission, yielding a visible color shift from yellow/green to blue. This behavior enabled the quantification of CA 19-9 over a linear range of 4-400 U mL^-1, with a limit of detection of

SUBMITTER: Rodrigues E 

PROVIDER: S-EPMC12694677 | biostudies-literature | 2025 Dec

REPOSITORIES: biostudies-literature