Local protein detection with lateral flow assay read through tissue using X-ray excited luminescence chemical imaging.

ABSTRACT:

Significance

Implant-associated infections can be difficult to detect at early stages. Noninvasively measuring inflammatory biomarkers such as C-reactive protein (CRP) in synovial fluid can improve early diagnosis and patient outcomes.

Aim

We aim to show proof of principle for an implantable immunoassay device combined with an optical readout method to detect extracellular proteins through tissue. The system integrates lateral flow assay (LFA) strips with X-ray scintillators in a sealed implantable casing.

Approach

An LFA strip is placed above a scintillator layer inside the implant. An X-ray beam excites the scintillator, generating luminescence that passes through the LFA. Test and control lines alter the transmitted light, and a fluorescent layer shifts the lumines

SUBMITTER: Ding Y 

PROVIDER: S-EPMC12694748 | biostudies-literature | 2025 Feb

REPOSITORIES: biostudies-literature