Extensive cultivation of human gut phages revealing undescribed <i>Bacteroidaceae</i> phages.

ABSTRACT: The isolated and characterized gut phages remain rare. Most of the gut phages that have been isolated and propagated thus far are lytic phages, leaving significant gaps in the study of gut temperate phages. In this study, we successfully isolated a large-scale collection of gut bacteria and phages, containing 1,679 bacterial strains from 86 species and 79 phages that infect bacteria from 26 different species. Among the phage isolates, 32 are temperate phages and two of these temperate phages were directly isolated from faecal samples of healthy human donors. Sequence comparisons and analysis show that the isolated temperate phages are characterized with highly diverse genomes and significantly higher prevalence in the human gut when compared with these characterised lytic gut phages. Furth

SUBMITTER: Liu Z 

PROVIDER: S-EPMC12694917 | biostudies-literature | 2025 Dec

REPOSITORIES: biostudies-literature