FBXO22 promotes hepatocellular carcinoma progression via paracrine myo‑inositol‑induced M2‑type polarization of macrophages.

ABSTRACT: Macrophages play a key role in hepatocellular carcinoma (HCC) progression, but the mechanisms underlying this involvement remain unclear. In the present study, mice with HCC were used for in vivo experiments, and 97H and THP‑1 cells were used for in vitro experiments. Metabolomic analysis was performed to detect changes of metabolites in the supernatant of 97H cells. Flow cytometry and immunohistochemical staining were performed to assess macrophage polarization. Western blotting was performed to examine the levels of phosphorylated (p‑) PI3K, p‑AKT and NRF2. Reverse transcription‑quantitative polymerase chain reaction was performed to examine FBXO22, IMPA1 and PTEN mRNA expression levels. FBXO22 significantly promoted the release of myo‑inositol in the c

SUBMITTER: Bai L 

PROVIDER: S-EPMC12695154 | biostudies-literature | 2026 Feb

REPOSITORIES: biostudies-literature