Intestinal dysbiosis in critically ill patients: a case-control study of <i>Enterobacteriaceae</i> enrichment and reduced microbial diversity.

ABSTRACT:

Introduction

Critical illness disrupts gut microbiota homeostasis, potentially exacerbating systemic inflammation and adverse outcomes. This study investigates gut dysbiosis patterns in ICU patients, with a focus on Enterobacteriaceae enrichment and microbial diversity loss, to identify biomarkers and therapeutic targets.

Methods

In this case-control study, 37 ICU patients (sepsis: n = 17; non-sepsis: n = 20) and 20 healthy controls were enrolled. Fecal samples underwent 16S rRNA sequencing (V3-V4 regions). Microbial diversity (Shannon/Simpson indices), beta diversity (Bray-Curtis PCoA), and taxonomic differences (LEfSe, LDA > 2.5) were analyzed using QIIME2 and R.

Results

Critically ill patients showed reduced alpha diversity vs. con

SUBMITTER: Wang D 

PROVIDER: S-EPMC12695524 | biostudies-literature | 2025

REPOSITORIES: biostudies-literature