M1 macrophage-derived exosomal miR-20b promotes radiosensitization via CCND1 in HPV⁺ HNSCC.

ABSTRACT:

Background

Human papillomavirus (HPV) is a significant risk factor for head and neck squamous cell carcinoma (HNSCC). HPV positive (HPV⁺) HNSCC is more sensitive to radiotherapy and has a better prognosis than HPV negative (HPV^-) HNSCC. M1 macrophages not only enhance the radiosensitivity of HPV+ HNSCC, but the M1 macrophages derived exosmoes (M1 exos) also possess anti-tumor activity. However, the role of M1 exos in the radiosensitivity of HNSCC remains unclear.

Materials and methods

HPV status and macrophage infiltration levels in 25 HNSCC tissues were evaluated by immunohistochemistry (IHC). M1 macrophages were induced and cultured in vitro, and exosomes were extracted through differential ultracentrifugation. The effect of M1 exos on the radi

SUBMITTER: Liu H 

PROVIDER: S-EPMC12695572 | biostudies-literature | 2025

REPOSITORIES: biostudies-literature