CU Cilia - an application for image analysis by machine learning - reveals significance of cysteine cathepsin K activity for primary cilia of human thyroid epithelial cells.

ABSTRACT:

Introduction

Cysteine cathepsins K and L are crucial for proper thyroid function in mice. Inhibition of cysteine peptidases in rodent thyroid epithelial cells in vitro results in loss of primary cilia, highlighting the significance of proteolysis for cilia maintenance.

Methods

In this study, we used an in vitro-approach to ask whether primary cilia of the normal human thyroid epithelial cell line Nthy-ori 3-1 are affected by cysteine cathepsin inhibition in numbers and/or structure. To interpret hundreds of primary cilia in dozens of high-resolution laser-scanning microscopic images, an objective image analysis approach is essential.

Results

Therefore, we developed CU Cilia, i.e., a method for the detection of primary cilia and for segmentation of

SUBMITTER: Rehders M 

PROVIDER: S-EPMC12695601 | biostudies-literature | 2025

REPOSITORIES: biostudies-literature