Nondestructive X-ray tomography of brain tissue ultrastructure.

ABSTRACT: Maps of biological tissues at subcellular detail are key for understanding how organs function. X-ray nanotomography is a promising alternative to volume electron microscopy: it has the potential to nondestructively image millimeter-sized samples at ultrastructural resolution within a few days. A fundamental barrier is that the intense X-rays required for imaging also deform and disintegrate the tissue samples. Here we show a combination of solutions that overcome this barrier: We used a cryogenic and stable sample stage, tailored nonrigid tomographic reconstruction algorithms and an epoxy resin developed for the nuclear and aerospace industry. Tissue samples were resistant to radiation doses exceeding 1.15 × 10¹⁰ Gy, and sub-40 nm isotropic resolution allowed identifying axon b

SUBMITTER: Bosch C 

PROVIDER: S-EPMC12695642 | biostudies-literature | 2025 Dec

REPOSITORIES: biostudies-literature