Reconstruction of a connectome of single neurons in mouse brains by cross-validating multi-scale multi-modality data.

ABSTRACT: Brain networks, or connectomes, have inspired research at macro-, meso- and micro-scales. However, the rise of single-cell technologies necessitates inferring connectomes consisting of individual neurons projecting throughout the brain. Her, we present a scalable approach to map single-neuron connectivity at the whole-brain scale using two complementary methods. We first generated an arbor-net by probabilistically pairing dendritic and axonal arbors of 20,247 neurons registered to the Allen Brain Atlas. We also produced a bouton-net based on 2.57 million putative axonal boutons from 1,877 fully reconstructed neurons and probabilistic pairing of these full-morphology datasets. Cross-validation of both networks showed statistical consistency in spatially and anatomically modular distribution

SUBMITTER: Xiong F 

PROVIDER: S-EPMC12695645 | biostudies-literature | 2025 Dec

REPOSITORIES: biostudies-literature