Origins of chromosome instability unveiled by coupled imaging and genomics.

ABSTRACT: Somatic chromosome instability results in widespread structural and numerical chromosomal abnormalities (CAs) during cancer evolution^1-3. Although CAs have been linked to mitotic errors resulting in the emergence of nuclear atypia^4-7, the underlying processes and rates of spontaneous CA formation in human cells are underexplored. Here we introduce machine-learning-assisted genomics and imaging convergence (MAGIC)-an autonomously operated platform that integrates live-cell imaging of micronucleated cells, machine learning on-the-fly and single-cell genomics to systematically investigate CA formation. Applying MAGIC to near-diploid, non-transformed cell lines, we track de novo CAs over successive cell cycles, highlighting the common role of dicentric chromosomes as init

SUBMITTER: Cosenza MR 

PROVIDER: S-EPMC12695650 | biostudies-literature | 2025 Dec

REPOSITORIES: biostudies-literature