Nitro-oleic acid activation of endothelial PPARγ signaling pathway alleviates neurovascular injury and improves functional outcomes in ischemic stroke.

ABSTRACT: Nitro-oleic acid (OA-NO₂) is an endogenous peroxisome proliferator-activated receptor-γ (PPARγ) ligand and can activate this receptor under both physiological and pathological conditions. In this study, we explore the role and molecular mechanisms of OA-NO₂ in maintaining blood-brain barrier (BBB) integrity and enhancing neurovascular function during ischemic stroke, with a particular emphasis on the activation of endothelial PPARγ signaling pathways. Endothelial cell-selective PPARγ conditional knockout (EC-PPARγ cKO) and wild-type (WT) mice underwent 1 h middle cerebral artery occlusion (MCAO) with 1-7 days of reperfusion. Mice were treated with oleic acid (OA) or OA-NO₂ (5 mg/kg) via tail vein 2 h after MCAO. Neurobehavioral deficits were assessed on day

SUBMITTER: Xiong T 

PROVIDER: S-EPMC12695680 | biostudies-literature | 2026 Jan

REPOSITORIES: biostudies-literature