Establishment of rapid saturation mutagenesis and screening methods for improving the neutralizing activity of monoclonal antibodies.

ABSTRACT:

Background

Monoclonal antibodies have been widely used in the fight against infectious diseases and are recommended for postexposure prophylaxis (PEP) of the rabies virus because of their advantages of easy supply, low cost, and high efficacy. To maximize treatment efficiency and minimize side effects, the binding affinity of isolated mAbs requires further enhancement.

Methods

In this study, we developed a high-throughput saturation mutagenesis platform to improve the neutralizing activity of a preclinical antirabies mAb. We generated a system displaying antibodies on mammalian cell surfaces to block the invasion of the pseudotyped rabies virus. Saturation mutagenesis was performed on the complementarity-determining regions (CDRs) of the parental mAb, followed by screening f

SUBMITTER: Wu X 

PROVIDER: S-EPMC12695841 | biostudies-literature | 2025

REPOSITORIES: biostudies-literature