Isolation and characterization of two novel phages with lytic activity against multidrug-resistant Acinetobacter baumannii strains: potential for phage therapy.

ABSTRACT: The emergence of multidrug-resistant Acinetobacter baumannii (MDR-AB) has raised concerns regarding the lack of effective treatment options, prompting interest in phage therapy. In this study, two phages, designated vB_MZM_2AB-P and vB_MZM_4AB-P, were isolated and characterized for their lytic activity against MDR-AB strains. vB_MZM_2AB-P demonstrated considerable stability, retaining activity at 70 °C and pH 11.00, with over 37.10% survival rate after three months of storage. It displayed a 10-min latent period and a burst size of 39.72 PFU/cell. vB_MZM_4AB-P was stable at 50 °C and pH 7.00, but lost activity after three months of storage. Its latent period was also 10 min, with a burst size as high as 746.70 PFU/cell. Transmission electron microscopy revealed that phage vB_MZM_2AB-P has

SUBMITTER: Chen X 

PROVIDER: S-EPMC12695905 | biostudies-literature | 2025 Dec

REPOSITORIES: biostudies-literature