Analysis of EST-SSR characteristics of prickly ash transcriptome and development of molecular markers.

ABSTRACT: To explore the genetic diversity and relationships among prickly ash germplasm resources in China, the number, distribution, and repeat types of simple sequence repeats (EST-SSR) loci were analyzed using the MISA software system based on the transcriptome data of prickly ash. Thirty-five prickly ash germplasms from nine provinces were selected, and 30 pairs of EST-SSR primers were randomly designed for polymorphism screening. The obtained polymorphic primers were used for genetic diversity assessment, to provide genomics theoretical support for the construction of molecular breeding system and sustainable utilization of germplasm resources of prickly ash.The results showed that from 245,286 Unigene sequences, 36,656 sequences contained 20,271 EST-SSR loci. The frequency of occurrence of ES

SUBMITTER: Liu J 

PROVIDER: S-EPMC12695933 | biostudies-literature | 2025 Dec

REPOSITORIES: biostudies-literature