Quantitative LFQ-DIA proteomics reveals FTH1-MCM5/WNT axis mediated osteoblastic dysfunction via ferroptosis drives diabetic osteoporosis.

ABSTRACT: In diabetes mellitus osteoporosis (DMOP), a common and severe chronic complication of diabetes mellitus (DM), long-term challenges are posed to public health. Recent evidence has implicated ferroptosis-a form of regulated cell death driven by iron-dependent lipid peroxidation-in the pathogenesis of DMOP. Ferritin heavy chain 1 (FTH1) plays a critical role in regulating iron metabolism during ferroptosis. To elucidate the regulatory mechanisms by which FTH1 modulates osteoblast (OB) ferroptosis and aberrant bone metabolism under high glucose and high fat (HGHF) conditions, we performed quantitative LFQ-DIA proteomics combined with bioinformatic analysis. MC3T3-E1 cells cultured under high glucose and high palmitic acid (HGPA) conditions were subjected to lentiviral-mediated FTH1 knock down

SUBMITTER: Lin J 

PROVIDER: S-EPMC12696055 | biostudies-literature | 2025 Dec

REPOSITORIES: biostudies-literature