Coculture Corynebacterium glutamicum and Bacillus licheniformis for producing poly-γ-glutamic acid from glucose.

ABSTRACT: Poly-γ-glutamic acid (γ-PGA) has diverse applications from cosmetic to drug delivery. The production of γ-PGA primarily relies on microbial fermentation using Bacillus spp. supplemented with L-glutamate supplementation. However, the high cost of L-glutamate supplementation limits industrial production. This study aimed to achieve direct γ-PGA production from glucose using a Corynebacterium glutamicum-Bacillus licheniformis coculture system. To create such a coculture system, we utilized B. licheniformis ATCC 9945a, a natural L-glutamate-dependent γ-PGA producing strain, and C. glutamicum F343, which exhibited an excellent capacity to produce L-glutamate from glucose. B. licheniformis ATCC 9945a grew well and produced small amounts of γ-PGA in the medium of C. glutamicum F343. Subsequently,

SUBMITTER: Liu W 

PROVIDER: S-EPMC12696065 | biostudies-literature | 2025 Dec

REPOSITORIES: biostudies-literature