Enhancing conjugation from E. coli to Streptomyces coelicolor by incorporating traJ into mobilizable plasmids.

ABSTRACT: Streptomyces species are prolific producers of bioactive natural products, yet their genetic manipulation remains constrained by inefficient DNA delivery methods in many strains. Conjugation from methylation-deficient Escherichia coli has become the preferred approach for introducing plasmids into Streptomyces, relying on the presence of the oriT sequence within the mobilizable plasmid and the conjugation machinery (tra genes) encoded on the non-mobilizable helper plasmid pUZ8002. Among these, traJ encodes an essential component of the relaxosome. An additional copy of traJ is present downstream of oriT in some mobilizable plasmids, whereas many other commonly used plasmids lack traJ. Here, we investigated the impact of including traJ in mobilizable plasmids on conjugation efficiency by en

SUBMITTER: Valdes-Chiara P 

PROVIDER: S-EPMC12696120 | biostudies-literature | 2025 Dec

REPOSITORIES: biostudies-literature